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Braz. j. microbiol ; 39(4): 605-607, Dec. 2008. ilus, tab
Article in English | LILACS | ID: lil-504295

ABSTRACT

The present study established a PCR protocol in order to identify Parvimonas micra and to evaluate the intra-species diversity by PCR-RFLP of 16S rRNA partial sequence. The data indicated that the protocol was able to identify this species which could be clustered in five genotypes.


O presente estudo estabeleceu um protocolo de PCR com a finalidade de identificar a espécie Parvimonas micra e avaliar a diversidade intra-espécie utilizando a técnica PCR-RFLP do gene que codifica o rRNA 16S. Os dados indicaram que o protocolo possibilitou a identificação da espécie e a distinção de 5 grupos genotípicos.


Subject(s)
Humans , Genetic Variation , In Vitro Techniques , Gram-Positive Bacterial Infections/genetics , Polymerase Chain Reaction , Peptostreptococcus/isolation & purification , RNA , Genetic Techniques , Genotype , Methods
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